How to run a query: 1) Go to services.bromberglab.org/mebipred/home 2) Enter the protein sequences on which you want to run MeBiPred. This can be done by the following: 2.1) Pasting them into the text box area 2.2) Clicking "Browse" to open a file browser where you can select a file for upload For both methods, the sequences must be in FASTA format 3) Click on the "Submit" button. If everything went well, a table should appear on the right side of the page. At this point, you can enter your email if you want to receive the results, but you will also have the option to open them through a link. 4) Click on the "Run" button. This will run MeBiPred on the uploaded sequences. The "Run" button should be replaced by text displaying the word "Running." 5) The webpage is set to auto refresh every 1 minute and check on the status of the job. Once the job has completed, a "Results" button should appear in the Actions column. Clicking on the "Results" button will display the results page.
How to use the result page: The top of the page displays a pie chart depicting the number of predictions for each ion. If a sequence is predicted to bind multiple ions, each prediction is counted independently. Below that, there is a table where each row displays the predictions for one sequence and each column displays the predictions for one ion. It is posible to search the name of an entry by entering text into the search box at the top-right corner of the table. You can also sort results by clicking on the header of the column you would like to sort by.